Journal: Scientific Reports

Article Title: Fabrication of a protein microarray by fluorous-fluorous interactions

doi: 10.1038/s41598-017-07571-4

Figure Lengend Snippet: Fluorous protein microarray for the simultaneous detection of multiple proteins. ( a ) MBP, GST, and anti-RCA 120 were printed on a fluorous slide. ( b ) Confirmation of the specific fluorous-fluorous interactions. Anti-RCA 120 , anti-RCA 120 pre-incubated with F tag -acid (compound 5), and anti-RCA 120 pre-incubated with F tag -BA (compound 2) solutions were spotted on a fluorous slide.

Article Snippet: The activity of fluorescent eGFP on the slide was directly measured with a NovaRay Microarray Scanner using a fluorescein isothiocyanate (FITC) filter.

Techniques: Microarray, Incubation

Journal:

Article Title: Genome-Wide Expression Profile Analysis Reveals Coordinately Regulated Genes Associated with Stepwise Acquisition of Azole Resistance in Candida albicans Clinical Isolates

doi: 10.1128/AAC.47.4.1220-1227.2003

Figure Lengend Snippet: (A) Cluster images showing genes differentially expressed in this series of isolates. Red represents genes that are up-regulated, and green represents genes that are down-regulated. Genes shown in both cluster images appear in the same order. Genes are grouped as being coordinately differentially expressed with CaMDR1 (I), CDR genes (II), neither CaMDR1 nor CDR genes (III), or both CaMDR1 and CDR genes (IV). (B) Confirmation of differential expression by RT-PCR of select genes found to be differentially expressed by cDNA microarray analysis. RT-PCR of C. albicans 18S rRNA was performed as a control.

Article Snippet: Slides were scanned using a ChipReader microarray scanner (Virtek Vision Intl.).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Microarray